by Ara Cho, Wencheng Jin, Jeonghwan Lee, Nayeon Shin, Myoung Seok Lee, Lilin Li, Seung Hee Yang, Kyong Soo Park, Chul Woo Yang, Dong Ki Kim, Yun Kyu Oh, Chun Soo Lim, Jung Pyo Lee
Abstract:
Abstract Diabetic nephropathy (DN) is associated with kidney fibrosis. A previous study revealed that periostin (POSTN) contributes to kidney fibrosis. This study examined the role of POSTN in DN. The urinary concentrations of POSTN and TNC increased according to the severity of DN in human samples. Streptozotocin (STZ) was administered after unilateral nephrectomy (UNXSTZ) to induce DN in wild-type and Postn -null mice. Four experimental groups were generated: wild-typeham (WT Sham), wild-type UNXSTZ (WT STZ), Postn- null Sham (KO Sham), and Postn -null UNXSTZ (KO STZ). After 20 weeks, the KO STZ group had lower levels of urine albumin excretion, glomerular sclerosis, and interstitial fibrosis than those of the WT STZ group. Additionally, the KO STZ group had lower expression of fibrosis markers, including TNC. The KO STZ group showed better glucose regulation than the WT STZ model. Furthermore, the KO STZ group exhibited significantly preserved pancreatic islet integrity and insulin expression. HK-2 cells were used to observe the aggravation of fibrosis caused by POSTN under TGF-β conditions. We stimulated INS-1 cells with streptozotocin and evaluated the viability of these cells. The anti-POSTN antibody treatment of INS-1 cells with streptozotocin resulted in higher cell viability than that with treatment with streptozotocin alone. The absence of POSTN in DN contributes to renal fibrosis alleviation by improving pancreatic β-cell function. Additionally, there is an association between POSTN and TNC.
Reference:
Ara Cho, Wencheng Jin, Jeonghwan Lee, Nayeon Shin, Myoung Seok Lee, Lilin Li, Seung Hee Yang, Kyong Soo Park, Chul Woo Yang, Dong Ki Kim, Yun Kyu Oh, Chun Soo Lim, Jung Pyo LeePeriostin deficiency attenuates kidney fibrosis in diabetic nephropathy by improving pancreatic β-cell dysfunction and reducing kidney EMTIn Scientific Reports, volume 13, 2023.
Bibtex Entry:
@article{cho_periostin_2023,
title = {Periostin deficiency attenuates kidney fibrosis in diabetic nephropathy by improving pancreatic β-cell dysfunction and reducing kidney {EMT}},
volume = {13},
issn = {2045-2322},
url = {https://www.nature.com/articles/s41598-023-44177-5},
doi = {10.1038/s41598-023-44177-5},
abstract = {Abstract
Diabetic nephropathy (DN) is associated with kidney fibrosis. A previous study revealed that periostin (POSTN) contributes to kidney fibrosis. This study examined the role of POSTN in DN. The urinary concentrations of POSTN and TNC increased according to the severity of DN in human samples. Streptozotocin (STZ) was administered after unilateral nephrectomy (UNXSTZ) to induce DN in wild-type and
Postn
-null mice. Four experimental groups were generated: wild-typeham (WT Sham), wild-type UNXSTZ (WT STZ),
Postn-
null Sham (KO Sham), and
Postn
-null UNXSTZ (KO STZ). After 20 weeks, the KO STZ group had lower levels of urine albumin excretion, glomerular sclerosis, and interstitial fibrosis than those of the WT STZ group. Additionally, the KO STZ group had lower expression of fibrosis markers, including TNC. The KO STZ group showed better glucose regulation than the WT STZ model. Furthermore, the KO STZ group exhibited significantly preserved pancreatic islet integrity and insulin expression. HK-2 cells were used to observe the aggravation of fibrosis caused by POSTN under TGF-β conditions. We stimulated INS-1 cells with streptozotocin and evaluated the viability of these cells. The anti-POSTN antibody treatment of INS-1 cells with streptozotocin resulted in higher cell viability than that with treatment with streptozotocin alone. The absence of POSTN in DN contributes to renal fibrosis alleviation by improving pancreatic β-cell function. Additionally, there is an association between POSTN and TNC.},
language = {en},
number = {1},
urldate = {2024-12-02},
journal = {Scientific Reports},
author = {Cho, Ara and Jin, Wencheng and Lee, Jeonghwan and Shin, Nayeon and Lee, Myoung Seok and Li, Lilin and Yang, Seung Hee and Park, Kyong Soo and Yang, Chul Woo and Kim, Dong Ki and Oh, Yun Kyu and Lim, Chun Soo and Lee, Jung Pyo},
month = oct,
year = {2023},
pages = {17599},
file = {Volltext:C:\Users\lovis\Zotero\storage\G9W8J55S\Cho et al. - 2023 - Periostin deficiency attenuates kidney fibrosis in.pdf:application/pdf},
}